Plasmid

Part:BBa_I713035:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pEB-EGFPL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1204
    Illegal XbaI site found at 1177
    Illegal PstI site found at 411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1204
    Illegal PstI site found at 411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1204
    Illegal BamHI site found at 1183
    Illegal XhoI site found at 426
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1204
    Illegal XbaI site found at 1177
    Illegal PstI site found at 411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1204
    Illegal XbaI site found at 1177
    Illegal PstI site found at 411
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1085
    Illegal BsaI.rc site found at 2520
    Illegal SapI site found at 1437


Design Notes

SphI-EGFP-SacI


Source

EGFP gene

References